产品描述

This Human GDNF ELISA Kit was designed for the quantitative measurement of Human GDNF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

别名

Astrocyte derived trophic factor ELISA Kit; Astrocyte derived trophic factor 1 ELISA Kit; Astrocyte-derived trophic factor ELISA Kit; ATF 1 ELISA Kit; ATF 2 ELISA Kit; Atf ELISA Kit; ATF1 ELISA Kit; ATF2 ELISA Kit; gdnf ELISA Kit; GDNF_HUMAN ELISA Kit; Glial cell derived neurotrophic factor ELISA Kit; Glial Cell Line Derived Neurotrophic Factor ELISA Kit; Glial cell line-derived neurotrophic factor ELISA Kit; Glial derived neurotrophic factor ELISA Kit; HFB1 GDNF ELISA Kit; hGDNF ELISA Kit; HSCR3 ELISA Kit

缩写

GDNF

Uniprot No

P39905

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human GDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		89-95
1:2		Average %		101
		Range %		97-105
1:4		Average %		92
		Range %		85-96
1:8		Average %		97
		Range %		91-100

回收率

The recovery of human GDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		90-98
EDTA plasma (n=4)		97		93-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   5000 2.066 1.952 2.009 1.848   2500 1.856 1.776 1.816 1.655   1250 1.542 1.523 1.533 1.372   625 1.122 1.137 1.130 0.969   312.5 0.709 0.684 0.697 0.536   156.25 0.478 0.459 0.469 0.308   78 0.296 0.304 0.300 0.139   0 0.163 0.159 0.161

货期

3-5 working days