产品描述
趋化因子13(C-X-C motif chemokine ligand 13)也称为BCA-1。它可以引导B淋巴细胞移动到淋巴结和其他组织,以便它们在那里激活和增殖。CXCL13在多种疾病中都发挥着重要作用,例如自身免疫性疾病、肿瘤和感染等。
华美生物所提供的Human B-Lymphocyte Chemoattractant 1,BLC-1 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的CXCL13,其灵敏度为19.5 pg/mL,检测范围为78 pg/mL-5000 pg/mL。
别名
ANGIE ELISA Kit; ANGIE2 ELISA Kit; B cell attracting chemokine 1 ELISA Kit; B cell-attracting chemokine 1 ELISA Kit; B lymphocyte chemoattractant ELISA Kit; B-cell chemoattractant ELISA Kit; B-cell-attracting chemokine 1 ELISA Kit; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1) ELISA Kit; BCA-1 ELISA Kit; BLC ELISA Kit; BLR1L ELISA Kit; C-X-C motif chemokine 13 ELISA Kit; Chemokine (C-X-C motif) ligand 13 ELISA Kit; Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant) ELISA Kit; Chemokine; CXC motif; ligand 13 ELISA Kit; CXC chemokine BLC ELISA Kit; CXCL13 ELISA Kit; CXL13_HUMAN ELISA Kit; SCYB13 ELISA Kit; Small inducible cytokine B subfamily (Cys-X-Cys motif); member 13 (B-cell chemoattractant) ELISA Kit; Small inducible cytokine B13 ELISA Kit; Small inducible cytokine subfamily B; member 13 ELISA Kit; Small-inducible cytokine B13 ELISA Kit
缩写
CXCL13
Uniprot No
O43927
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human BLC-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
86-99 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-100 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:8 |
Average % |
92 |
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| Range % |
83-104 |
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回收率
| The recovery of human BLC-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
90-105 |
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| EDTA plasma (n=4) |
92 |
88-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.804 |
2.856 |
2.830 |
2.661 |
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| 2500 |
2.132 |
2.047 |
2.090 |
1.921 |
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| 1250 |
1.339 |
1.346 |
1.343 |
1.174 |
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| 625 |
0.823 |
0.839 |
0.831 |
0.662 |
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| 312 |
0.476 |
0.484 |
0.480 |
0.311 |
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| 156 |
0.326 |
0.342 |
0.334 |
0.165 |
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| 78 |
0.247 |
0.251 |
0.249 |
0.080 |
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| 0 |
0.167 |
0.170 |
0.169 |
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货期
3-5 working days