产品描述
This Bovine IFNG ELISA Kit was designed for the quantitative measurement of Bovine IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
IFNG ELISA Kit; Interferon gamma ELISA Kit; BoIFNG ELISA Kit; IFN-gamma ELISA Kit
缩写
IFNG
Uniprot No
P07353
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
86-95 |
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| 1:2 |
Average % |
102 |
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| Range % |
99-105 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-97 |
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| 1:8 |
Average % |
97 |
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| Range % |
93-101 |
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回收率
| The recovery of bovine IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-96 |
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| EDTA plasma (n=4) |
95 |
92-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
1.974 |
1.882 |
1.928 |
1.785 |
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| 1000 |
1.290 |
1.196 |
1.243 |
1.100 |
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| 500 |
0.818 |
0.807 |
0.813 |
0.670 |
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| 250 |
0.519 |
0.502 |
0.511 |
0.368 |
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| 125 |
0.324 |
0.333 |
0.329 |
0.186 |
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| 62.5 |
0.268 |
0.280 |
0.274 |
0.131 |
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| 31.25 |
0.212 |
0.221 |
0.217 |
0.074 |
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| 0 |
0.142 |
0.144 |
0.143 |
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货期
3-5 working days