产品描述
This Human HBB ELISA Kit was designed for the quantitative measurement of Human HBB protein in serum, plasma, lysate for RBC. It is a Competitive ELISA kit, its detection range is 0.12 ng/mL-500 ng/mL and the sensitivity is 0.12 ng/mL.
别名
Beta-globin ELISA Kit; HBB ELISA Kit; HBB_HUMAN ELISA Kit; Hemoglobin alpha chain ELISA Kit; Hemoglobin beta chain ELISA Kit; heterotetrameric hemoglobin ELISA Kit; Spinorphin ELISA Kit; subunit alpha ELISA Kit
缩写
HBB
Uniprot No
P68871
灵敏度
0.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HB-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:2 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:4 |
Average % |
90 |
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| Range % |
88-94 |
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| 1:8 |
Average % |
88 |
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| Range % |
84-92 |
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回收率
| The recovery of human HB-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
105 |
100-113 |
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| EDTA plasma (n=4) |
98 |
93-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 500 |
0.295 |
0.280 |
0.288 |
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| 250 |
0.384 |
0.368 |
0.376 |
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| 125 |
0.508 |
0.487 |
0.498 |
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| 62.5 |
0.621 |
0.639 |
0.630 |
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| 31.2 |
0.794 |
0.822 |
0.808 |
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| 15.6 |
1.447 |
1.473 |
1.460 |
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| 7.8 |
1.849 |
1.801 |
1.825 |
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| 0 |
2.318 |
2.452 |
2.385 |
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货期
3-5 working days