产品描述

This Human EFNA1 ELISA Kit was designed for the quantitative measurement of Human EFNA1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

B61 ELISA Kit; ECKLG ELISA Kit; EFL 1 ELISA Kit; EFL1 ELISA Kit; EFNA 1 ELISA Kit; Efna1 ELISA Kit; EFNA1_HUMAN ELISA Kit; EPH related receptor tyrosine kinase ligand 1 ELISA Kit; EPH-related receptor tyrosine kinase ligand 1 ELISA Kit; Ephrin-A1 ELISA Kit; Ephrin-A1, secreted form ELISA Kit; EphrinA1 ELISA Kit; EPLG 1 ELISA Kit; EPLG1 ELISA Kit; Immediate early response protein B61 ELISA Kit; LERK 1 ELISA Kit; LERK-1 ELISA Kit; LERK1 ELISA Kit; Ligand of eph related kinase 1 ELISA Kit; OTTHUMP00000033242 ELISA Kit; OTTHUMP00000033271 ELISA Kit; secreted form ELISA Kit; TNF alpha-induced protein 4 ELISA Kit; TNFAIP 4 ELISA Kit; TNFAIP4 ELISA Kit; Tumor necrosis factor alpha induced protein 4 ELISA Kit; Tumor necrosis factor alpha-induced protein 4 ELISA Kit

缩写

EFNA1

Uniprot No

P20827

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of Human EFNA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		84-92
1:2		Average %		96
		Range %		90-102
1:4		Average %		93
		Range %		85-97
1:8		Average %		88
		Range %		82-94

回收率

The recovery of Human EFNA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		87-97
EDTA plasma (n=4)		86		82-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.918 2.717 2.818 2.701 5 2.425 2.524 2.475 2.358 2.5 1.913 1.816 1.865 1.748 1.25 1.130 1.182 1.156 1.039 0.625 0.580 0.547 0.564 0.447 0.312 0.345 0.331 0.338 0.221 0.156 0.207 0.211 0.209 0.092 0 0.116 0.117 0.117

货期

3-5 working days