产品描述
This Human IFNB1 ELISA Kit was designed for the quantitative measurement of Human IFNB1 protein in serum, urine, cell culture supernates, tissue homogenates, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
beta-interferon ELISA Kit; Fibroblast interferon ELISA Kit; IFB ELISA Kit; IFF ELISA Kit; IFN beta ELISA Kit; IFN-beta ELISA Kit; IFNB 1 ELISA Kit; IFNB ELISA Kit; IFNB_HUMAN ELISA Kit; IFNB1 ELISA Kit; Interferon beta 1 fibroblast ELISA Kit; Interferon beta ELISA Kit; Interferon beta precursor ELISA Kit; MGC96956 ELISA Kit
缩写
IFNB1
Uniprot No
P01574
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 84 | |
| Range % | 80-89 | |
| 1:2 | Average % | 100 | |
| Range % | 91-110 | |
| 1:4 | Average % | 97 | |
| Range % | 92-105 | |
| 1:8 | Average % | 93 | |
| Range % | 89-98 | |
回收率
| The recovery of human IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 89-96 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.523 | 2.416 | 2.470 | 2.356 | | | 1000 | 2.214 | 2.128 | 2.171 | 2.057 | | | 500 | 1.803 | 1.724 | 1.764 | 1.650 | | | 250 | 1.261 | 1.225 | 1.243 | 1.129 | | | 125 | 0.765 | 0.716 | 0.741 | 0.627 | | | 62.5 | 0.463 | 0.445 | 0.454 | 0.340 | | | 31.25 | 0.289 | 0.274 | 0.282 | 0.168 | | | 0 | 0.115 | 0.113 | 0.114 | | |
|
货期
3-5 working days