产品描述
This Human GAA ELISA Kit was designed for the quantitative measurement of Human GAA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.
别名
70 kDa lysosomal alpha-glucosidase ELISA Kit; Acid alpha glucosidase ELISA Kit; Acid maltase ELISA Kit; Aglucosidase alfa ELISA Kit; Alpha glucosidase ELISA Kit; GAA ELISA Kit; Glucosidase alpha acid (Pompe disease glycogen storage disease type II) ELISA Kit; Glucosidase alpha acid ELISA Kit; Glucosidase alpha ELISA Kit; LYAG ELISA Kit; LYAG_HUMAN ELISA Kit; Lysosomal alpha glucosidase ELISA Kit
缩写
GAA
Uniprot No
P10253
灵敏度
6.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 91-97 | |
| 1:2 | Average % | 84 | |
| Range % | 81-89 | |
| 1:4 | Average % | 99 | |
| Range % | 95-104 | |
| 1:8 | Average % | 93 | |
| Range % | 90-96 | |
回收率
| The recovery of human GAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 83-90 | |
| EDTA plasma (n=4) | 86 | 83-89 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1600 | 2.364 | 2.547 | 2.456 | 2.323 | | | 800 | 1.596 | 1.568 | 1.582 | 1.449 | | | 400 | 1.001 | 1.035 | 1.018 | 0.885 | | | 200 | 0.612 | 0.634 | 0.623 | 0.490 | | | 100 | 0.413 | 0.416 | 0.415 | 0.282 | | | 50 | 0.288 | 0.276 | 0.282 | 0.149 | | | 25 | 0.197 | 0.173 | 0.185 | 0.052 | | | 0 | 0.131 | 0.135 | 0.133 | | |
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货期
3-5 working days