产品描述
免疫球蛋白D(Immunoglobulin D,IgD)是人体中分布较少的一种免疫球蛋白,其主要存在于B淋巴细胞的表面,参与机体免疫应答的调节。IgD通过B细胞表面的抗原受体进行信号传递,促进B细胞的激活和增殖,是体液免疫和细胞免疫的重要组成部分。IgD的水平变化与多种疾病相关,如免疫系统异常、感染、过敏反应等。
华美生物所提供的Human Immunoglobulin D(IgD) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的IgD,其灵敏度为0.024 ng/mL,检测范围为0.024 ng/mL-100 ng/mL。
别名
N/A
缩写
IgD
灵敏度
0.024 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IgD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
91 |
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| Range % |
87-98 |
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| 1:200 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:400 |
Average % |
93 |
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| Range % |
87-99 |
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| 1:800 |
Average % |
94 |
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| Range % |
86-105 |
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回收率
| The recovery of human IgD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-96 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.120 |
2.094 |
2.107 |
2.005 |
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| 25 |
1.655 |
1.637 |
1.646 |
1.544 |
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| 6.25 |
0.995 |
1.005 |
1.000 |
0.898 |
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| 1.56 |
0.515 |
0.507 |
0.511 |
0.409 |
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| 0.39 |
0.413 |
0.408 |
0.411 |
0.309 |
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| 0.1 |
0.204 |
0.210 |
0.207 |
0.105 |
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| 0.024 |
0.138 |
0.132 |
0.135 |
0.033 |
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| 0 |
0.100 |
0.103 |
0.102 |
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货期
3-5 working days