产品描述
脂肪酸是一类重要的生物分子,是脂质的主要组成部分。脂肪酸可以作为能量来源,也可以被用于合成脂类分子如三酰甘油和磷脂。不同长度的脂肪酸和不饱和度对生物体的健康有着不同的影响,如Omega-3脂肪酸对心血管健康有益。脂肪酸代谢异常也与多种疾病有关,如肥胖、2型糖尿病和心脑血管疾病等。
华美生物所提供的Rat Folic acid,FA ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆样本中的FA,其灵敏度为19.5 pg/mL,检测范围为19.5 pg/mL-5000 pg/mL。
别名
N/A
缩写
FA
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat folic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
86-104 |
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| 1:2 |
Average % |
92 |
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| Range % |
83-99 |
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| 1:4 |
Average % |
96 |
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| Range % |
87-98 |
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| 1:8 |
Average % |
94 |
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| Range % |
80-98 |
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回收率
| The recovery of rat folic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
86-102 |
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| EDTA plasma (n=4) |
94 |
88-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 5000 |
0.324 |
0.326 |
0.325 |
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| 1250 |
0.514 |
0.528 |
0.521 |
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| 312 |
0.817 |
0.814 |
0.816 |
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| 78 |
1.281 |
1.293 |
1.287 |
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| 19.5 |
1.674 |
1.635 |
1.655 |
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| 0 |
1.948 |
1.966 |
1.957 |
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货期
3-5 working days