产品描述
This Human EZR ELISA Kit was designed for the quantitative measurement of Human EZR protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Villin 2 ezrin ELISA Kit; CVIL ELISA Kit; CVL ELISA Kit; Cytovillin 2 ELISA Kit; Cytovillin ELISA Kit; DKFZp762H157 ELISA Kit; Epididymis secretory protein Li 105 ELISA Kit; EZR ELISA Kit; EZRI_HUMAN ELISA Kit; Ezrin ELISA Kit; FLJ26216 ELISA Kit; HEL S 105 ELISA Kit; MGC1584 ELISA Kit; p81 ELISA Kit; VIL 2 ELISA Kit; VIL2 ELISA Kit; Villin 2 (ezrin) ELISA Kit; Villin 2 ELISA Kit; Villin-2 ELISA Kit; Villin2 ELISA Kit
缩写
EZR
Uniprot No
P15311
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human cytovillin/ezrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 91-101 | |
| 1:2 | Average % | 100 | |
| Range % | 95-104 | |
| 1:4 | Average % | 94 | |
| Range % | 87-97 | |
| 1:8 | Average % | 98 | |
| Range % | 93-103 | |
回收率
| The recovery of human cytovillin/ezrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 88-95 | |
| EDTA plasma (n=4) | 95 | 89-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 1.710 | 1.754 | 1.732 | 1.603 | | | 10 | 1.183 | 1.167 | 1.175 | 1.046 | | | 5 | 0.749 | 0.755 | 0.752 | 0.623 | | | 2.5 | 0.377 | 0.366 | 0.372 | 0.243 | | | 1.25 | 0.292 | 0.308 | 0.300 | 0.171 | | | 0.625 | 0.222 | 0.237 | 0.230 | 0.101 | | | 0.312 | 0.175 | 0.166 | 0.171 | 0.042 | | | 0 | 0.128 | 0.130 | 0.129 | | |
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货期
3-5 working days