产品描述
IFNG(干扰素γ,Interferon-γ)是由免疫细胞(如NK细胞和T细胞)分泌的细胞因子,具有抗病毒、免疫调节和免疫效应增强等多种生物学功能。它可以刺激免疫细胞产生炎症反应,增强巨噬细胞、T细胞和B细胞的活性,促进细胞因子产生。因此,IFNG在抵抗感染、抗肿瘤、自身免疫性疾病等方面都具有重要的作用。
华美生物所提供的Mouse Interferon γ ,IFN-γ ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆、细胞培养上清样本中的IFNG,其灵敏度为3.9 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
Ifng ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写
IFNG
Uniprot No
P01580
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
82-99 |
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| 1:2 |
Average % |
97 |
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| Range % |
87-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
90 |
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| Range % |
82-98 |
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回收率
| The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-93 |
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| EDTA plasma (n=4) |
99 |
92-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.889 |
2.785 |
2.837 |
2.670 |
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| 500 |
2.356 |
2.324 |
2.340 |
2.173 |
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| 250 |
1.571 |
1.614 |
1.593 |
1.426 |
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| 125 |
0.885 |
0.862 |
0.874 |
0.707 |
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| 62.5 |
0.569 |
0.542 |
0.556 |
0.389 |
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| 31.2 |
0.315 |
0.330 |
0.323 |
0.156 |
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| 15.6 |
0.254 |
0.247 |
0.251 |
0.084 |
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| 0 |
0.164 |
0.169 |
0.167 |
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货期
3-5 working days