产品描述

This Human CYP450 ELISA Kit was designed for the quantitative measurement of Human CYP450 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

AHH ELISA Kit; AHRR ELISA Kit; Aryl hydrocarbon hydroxylase ELISA Kit; CP11 ELISA Kit; CP1A1_HUMAN ELISA Kit; CYP 1 ELISA Kit; CYP1 ELISA Kit; cyp1a1 ELISA Kit; CYPIA1 ELISA Kit; Cytochrome P1 450 dioxin inducible ELISA Kit; Cytochrome P1-450 ELISA Kit; Cytochrome P450 1A1 ELISA Kit; Cytochrome P450 family 1 subfamily A polypeptide 1 ELISA Kit; Cytochrome P450 form 6 ELISA Kit; Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 ELISA Kit; Cytochrome P450-C ELISA Kit; Cytochrome P450-P1 ELISA Kit; Flavoprotein-linked monooxygenase ELISA Kit; Microsomal monooxygenase ELISA Kit; P1 450 ELISA Kit; P450 C ELISA Kit; P450 form 6 ELISA Kit; P450 P1 ELISA Kit; P450DX ELISA Kit; Xenobiotic monooxygenase ELISA Kit

缩写

CYP450

Uniprot No

P04798

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Cytochrome P450 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		88-97
1:2		Average %		104
		Range %		100-112
1:4		Average %		95
		Range %		90-100
1:8		Average %		84
		Range %		80-89

回收率

The recovery of human Cytochrome P450 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		94-102
EDTA plasma (n=4)		89		84-93

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 2000 2.407 2.532 2.470 2.319 1000 1.611 1.684 1.648 1.497 500 1.052 1.005 1.029 0.878 250 0.609 0.593 0.601 0.450 125 0.395 0.419 0.407 0.256 62.5 0.313 0.326 0.320 0.169 31.25 0.268 0.251 0.260 0.109 0 0.154 0.147 0.151

货期

3-5 working days