产品描述

This Human IGF2BP3 ELISA Kit was designed for the quantitative measurement of Human IGF2BP3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 37.5 pg/mL-2400 pg/mL and the sensitivity is 9.37 pg/mL.

别名

Cancer/testis antigen 98 ELISA Kit; CT98 ELISA Kit; DKFZp686F1078 ELISA Kit; hKOC ELISA Kit; IF2B3_HUMAN ELISA Kit; IGF II mRNA binding protein 3 ELISA Kit; IGF-II mRNA-binding protein 3 ELISA Kit; IGF2 mRNA binding protein 3 ELISA Kit; IGF2 mRNA-binding protein 3 ELISA Kit; IGF2BP3 ELISA Kit; IMP 3 ELISA Kit; IMP-3 ELISA Kit; Insulin like growth factor 2 mRNA binding protein 3 ELISA Kit; Insulin-like growth factor 2 mRNA-binding protein 3 ELISA Kit; KH domain containing protein overexpressed in cancer ELISA Kit; KH domain-containing protein overexpressed in cancer ELISA Kit; KOC 1 ELISA Kit; KOC1 ELISA Kit; VICKZ 3 ELISA Kit; VICKZ family member 3 ELISA Kit; VICKZ3 ELISA Kit

缩写

IGF2BP3

Uniprot No

O00425

灵敏度

9.37 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-95
1:2		Average %		102
		Range %		97-107
1:4		Average %		91
		Range %		85-97
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human IGF2BP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-96
EDTA plasma (n=4)		97		93-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2400 2.784 2.799 2.792 2.640 1200 1.956 1.906 1.931 1.779 600 1.095 1.123 1.109 0.957 300 0.637 0.665 0.651 0.499 150 0.419 0.451 0.435 0.283 75 0.338 0.342 0.340 0.188 37.5 0.247 0.268 0.258 0.106 0 0.151 0.153 0.152

货期

3-5 working days