产品描述
This Goat IFNG ELISA Kit was designed for the quantitative measurement of Goat IFNG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写
IFNG
Uniprot No
P79154
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 87-96 | |
| 1:2 | Average % | 103 | |
| Range % | 99-107 | |
| 1:4 | Average % | 93 | |
| Range % | 89-98 | |
| 1:8 | Average % | 92 | |
| Range % | 89-94 | |
回收率
| The recovery of goat IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 93-102 | |
| EDTA plasma (n=4) | 91 | 88-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.833 | 2.998 | 2.916 | 2.818 | | | 250 | 2.257 | 2.325 | 2.291 | 2.193 | | | 125 | 1.742 | 1.795 | 1.769 | 1.671 | | | 62.5 | 1.111 | 1.118 | 1.115 | 1.017 | | | 31.2 | 0.754 | 0.784 | 0.769 | 0.671 | | | 15.6 | 0.377 | 0.410 | 0.394 | 0.296 | | | 7.8 | 0.268 | 0.251 | 0.260 | 0.162 | | | 0 | 0.101 | 0.094 | 0.098 | | |
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货期
3-5 working days