产品描述
This Mouse HIF1A ELISA Kit was designed for the quantitative measurement of Mouse HIF1A protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL .
别名
Hif1aHypoxia-inducible factor 1-alpha ELISA Kit; HIF-1-alpha ELISA Kit; HIF1-alpha ELISA Kit; ARNT-interacting protein ELISA Kit
缩写
HIF1A
Uniprot No
Q61221
灵敏度
0.156 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HIF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 100 | |
| Range % | 95-107 | |
| 1:200 | Average % | 89 | |
| Range % | 83-98 | |
| 1:400 | Average % | 91 | |
| Range % | 87-95 | |
| 1:800 | Average % | 96 | |
| Range % | 90-101 | |
回收率
| The recovery of mouse HIF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 87-95 | |
| EDTA plasma (n=4) | 94 | 90-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.253 | 2.233 | 2.243 | 2.186 | | | 20 | 1.346 | 1.275 | 1.311 | 1.254 | | | 10 | 0.792 | 0.752 | 0.772 | 0.715 | | | 5 | 0.468 | 0.491 | 0.480 | 0.423 | | | 2.5 | 0.263 | 0.264 | 0.264 | 0.207 | | | 1.25 | 0.222 | 0.240 | 0.231 | 0.174 | | | 0.625 | 0.118 | 0.122 | 0.120 | 0.063 | | | 0 | 0.055 | 0.058 | 0.057 | | |
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货期
3-5 working days