产品描述
This Human FTL ELISA Kit was designed for the quantitative measurement of Human FTL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .
别名
Ferritin L chain ELISA Kit; Ferritin L subunit ELISA Kit; Ferritin light chain ELISA Kit; Ferritin light polypeptide ELISA Kit; ferritin light polypeptide like 3 ELISA Kit; FRIL_HUMAN ELISA Kit; FTL ELISA Kit; LFTD ELISA Kit; NBIA 3 ELISA Kit; NBIA3 ELISA Kit
缩写
FTL
Uniprot No
P02792
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human FTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 87-95 | |
| 1:2 | Average % | 94 | |
| Range % | 90-98 | |
| 1:4 | Average % | 94 | |
| Range % | 89-97 | |
| 1:8 | Average % | 100 | |
| Range % | 93-103 | |
回收率
| The recovery of human FTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 90-104 | |
| EDTA plasma (n=4) | 93 | 89-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 3.055 | 3.167 | 3.111 | 2.987 | | | 20 | 2.389 | 2.288 | 2.339 | 2.215 | | | 10 | 1.413 | 1.429 | 1.421 | 1.297 | | | 5 | 0.857 | 0.842 | 0.850 | 0.726 | | | 2.5 | 0.462 | 0.483 | 0.473 | 0.349 | | | 1.25 | 0.320 | 0.334 | 0.327 | 0.203 | | | 0.625 | 0.245 | 0.251 | 0.248 | 0.124 | | | 0 | 0.122 | 0.126 | 0.124 | | |
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货期
3-5 working days