产品描述
This Human F11 ELISA Kit was designed for the quantitative measurement of Human F11 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.
别名
coagulation factor XI ELISA Kit; Coagulation factor XIa light chain ELISA Kit; F11 ELISA Kit; FA11_HUMAN ELISA Kit; FXI ELISA Kit; MGC141891 ELISA Kit; Plasma thromboplastin antecedent ELISA Kit; Platelet coagulation factor XI ELISA Kit; PTA ELISA Kit
缩写
F11
Uniprot No
P03951
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Blood Coagulation
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human F11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1000 | Average % | 85 | | |
| Range % | 82-88 | | |
| 1:2000 | Average % | 110 | | |
| Range % | 107-113 | | |
| 1:4000 | Average % | 91 | | |
| Range % | 85-97 | | |
| 1:8000 | Average % | 92 | | |
| Range % | 87-95 | | |
回收率
| The recovery of human F11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 102 | 98-106 | | |
| EDTA plasma (n=4) | 86 | 82-92 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 5000 | 2.704 | 2.681 | 2.693 | 2.617 | | | | 2500 | 2.092 | 2.138 | 2.115 | 2.039 | | | | 1250 | 1.387 | 1.405 | 1.396 | 1.320 | | | | 625 | 0.845 | 0.868 | 0.857 | 0.781 | | | | 312 | 0.503 | 0.538 | 0.521 | 0.445 | | | | 156 | 0.301 | 0.296 | 0.299 | 0.223 | | | | 78 | 0.176 | 0.186 | 0.181 | 0.105 | | | | 0 | 0.077 | 0.074 | 0.076 | | | |
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货期
3-5 working days