产品描述
This Fish HSP70 ELISA Kit was designed for the quantitative measurement of Fish HSP70 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 18.75 pg/mL-300 pg/mL and the sensitivity is 18.75 pg/mL.
别名
Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit
缩写
HSP-70
Uniprot No
Q9I8F9
灵敏度
18.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of fish HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-95 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
82 |
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| Range % |
80-85 |
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| 1:8 |
Average % |
91 |
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| Range % |
83-99 |
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回收率
| The recovery of fish HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-97 |
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| EDTA plasma (n=4) |
94 |
91-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 300 |
0.153 |
0.144 |
0.149 |
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| 150 |
0.255 |
0.242 |
0.249 |
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| 75 |
0.519 |
0.533 |
0.526 |
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| 37.5 |
1.103 |
1.134 |
1.119 |
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| 18.75 |
2.363 |
2.270 |
2.317 |
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货期
3-5 working days