产品描述
This Human FABP1 ELISA Kit was designed for the quantitative measurement of Human FABP1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.2 pg/mL-2000pg/mL and the sensitivity is 7.8 pg/mL.
别名
FABP 1 ELISA Kit; FABP1 ELISA Kit; FABPL ELISA Kit; FABPL_HUMAN ELISA Kit; Fatty Acid Binding Protein 1 ELISA Kit; Fatty acid binding protein 1 liver ELISA Kit; Fatty Acid Binding Protein ELISA Kit; Fatty acid-binding protein 1 ELISA Kit; Fatty acid-binding protein ELISA Kit; Fatty acid-binding protein liver ELISA Kit; L FABP ELISA Kit; L-FABP ELISA Kit; liver ELISA Kit; Liver-type fatty acid-binding protein ELISA Kit
缩写
FABP1
Uniprot No
P07148
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human L-FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 86 | |
| Range % | 80-92 | |
| 1:200 | Average % | 98 | |
| Range % | 91-105 | |
| 1:400 | Average % | 98 | |
| Range % | 92-110 | |
| 1:800 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of human L-FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 96 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.716 | 2.652 | 2.684 | 2.604 | | | 1000 | 1.690 | 1.676 | 1.683 | 1.603 | | | 500 | 1.049 | 1.002 | 1.026 | 0.946 | | | 250 | 0.566 | 0.539 | 0.553 | 0.473 | | | 125 | 0.342 | 0.324 | 0.333 | 0.253 | | | 62.5 | 0.208 | 0.199 | 0.204 | 0.124 | | | 31.25 | 0.148 | 0.138 | 0.143 | 0.063 | | | 0 | 0.083 | 0.077 | 0.080 | | |
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货期
3-5 working days