产品描述
The rat CXCR4 ELISA Kit is used to quantitatively measure rat CXCR4 levels in serum, plasma, cell culture supernates, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of CXCR4 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
CXCR4 binds to SDF-1, transducing a cascade downstream signaling including PI3K/AKT, mTOR, and NF-κB pathways that eventually regulate cell survival, proliferation, chemotaxis, apoptosis and differentiation, and enhance angiogenesis in targeted diseases. CXCR4 plays a pivotal role in both physiological processes such as germ cell development, neurogenesis, vascular formation, and cardiogenesis and pathological processes such as muscle regeneration and vascular formation. Upregulation of CXCR4 can be found during injury, hypoxia, stress, and vascular tissue damage.
别名
Cxcr4; Cmkar4; C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; Fusin; Leukocyte-derived seven transmembrane domain receptor; LESTR; Stromal cell-derived factor 1 receptor; SDF-1 receptor; CD antigen CD184
缩写
CXCR4
Uniprot No
O08565
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CXCR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 86-95 | |
| 1:2 | Average % | 103 | |
| Range % | 97-106 | |
| 1:4 | Average % | 93 | |
| Range % | 85-96 | |
| 1:8 | Average % | 97 | |
| Range % | 91-100 | |
回收率
| The recovery of rat CXCR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 96 | 92-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 1.868 | 1.856 | 1.862 | 1.775 | | | 5 | 1.133 | 1.122 | 1.128 | 1.041 | | | 2.5 | 0.742 | 0.757 | 0.750 | 0.663 | | | 1.25 | 0.548 | 0.532 | 0.540 | 0.453 | | | 0.625 | 0.326 | 0.312 | 0.319 | 0.232 | | | 0.312 | 0.224 | 0.218 | 0.221 | 0.134 | | | 0.156 | 0.174 | 0.185 | 0.180 | 0.093 | | | 0 | 0.088 | 0.086 | 0.087 | | |
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货期
3-5 working days
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