产品描述

This Human DNAJC19 ELISA Kit was designed for the quantitative measurement of Human DNAJC19 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL.

别名

DnaJ (Hsp40) homolog; subfamily C; member 19 ELISA Kit; DnaJ homolog subfamily C member 19 ELISA Kit; DNAJC19 ELISA Kit; homolog of yeast TIM14 ELISA Kit; Mitochondrial import inner membrane translocase subunit TIM14 ELISA Kit; PAM18 ELISA Kit; TIM 14 ELISA Kit; TIM14 ELISA Kit; TIM14_HUMAN ELISA Kit; TIMM 14 ELISA Kit; TIMM14 ELISA Kit; translocase of the inner mitochondrial membrane 14 ELISA Kit; Translocase of the inner mitochondrial membrane 14; yeast homolog of ELISA Kit

缩写

DNAJC19

Uniprot No

Q96DA6

灵敏度

7 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human DNAJC19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		92-101
1:2		Average %		86
		Range %		81-90
1:4		Average %		93
		Range %		89-98
1:8		Average %		90
		Range %		85-94

回收率

The recovery of human DNAJC19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		99-107
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1800 2.656 2.719 2.688 2.579 900 2.287 2.172 2.230 2.121 450 1.653 1.707 1.680 1.571 225 1.135 1.071 1.103 0.994 112.5 0.681 0.653 0.667 0.558 56 0.374 0.362 0.368 0.259 28 0.257 0.252 0.255 0.146 0 0.112 0.105 0.109

货期

3-5 working days