产品描述

This Human IFIT1 ELISA Kit was designed for the quantitative measurement of Human IFIT1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

C56 ELISA Kit; G10P1 ELISA Kit; GARG 16 ELISA Kit; GARG16 ELISA Kit; IFI 56 ELISA Kit; IFI 56K ELISA Kit; IFI-56K ELISA Kit; IFI56 ELISA Kit; IFIT-1 ELISA Kit; Ifit1 ELISA Kit; IFIT1_HUMAN ELISA Kit; IFNAI1 ELISA Kit; Interferon alpha inducible protein (MW 56kD) ELISA Kit; Interferon induced 56 kDa protein ELISA Kit; Interferon induced protein 56 ELISA Kit; Interferon inducible mRNA 561 ELISA Kit; Interferon-induced 56 kDa protein ELISA Kit; Interferon-induced protein with tetratricopeptide repeats 1 ELISA Kit; ISG56 ELISA Kit; OTTHUMP00000020062 ELISA Kit; P56 ELISA Kit; RNM561 ELISA Kit

缩写

IFIT1

Uniprot No

P09914

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IFIT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		89-98
1:2		Average %		98
		Range %		93-103
1:4		Average %		88
		Range %		84-92
1:8		Average %		102
		Range %		97-108

回收率

The recovery of human IFIT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		101		96-105
EDTA plasma (n=4)		89		83-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1000 2.835 2.986 2.911 2.770 500 2.183 2.295 2.239 2.098 250 1.321 1.397 1.359 1.218 125 0.702 0.714 0.708 0.567 62.5 0.453 0.435 0.444 0.303 31.25 0.277 0.285 0.281 0.140 15.6 0.229 0.224 0.227 0.086 0 0.140 0.142 0.141

货期

3-5 working days