产品描述
This Human FAM132A ELISA Kit was designed for the quantitative measurement of Human FAM132A protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .
别名
C1QTNF12 ELISA Kit; C1QDC2 ELISA Kit; CTRP12 ELISA Kit; FAM132AAdipolin ELISA Kit; Adipose-derived insulin-sensitizing factor ELISA Kit; C1q and TNF related protein 12 ELISA Kit; Complement C1q tumor necrosis factor-related protein 12) [Cleaved into: Adipolin fC1QTNF12 ELISA Kit; Adipolin fCTRP12 ELISA Kit; Adipolin full-length form); Adipolin gC1QTNF12 ELISA Kit; Adipolin cleaved form ELISA Kit; Adipolin gCTRP12)] ELISA Kit
缩写
FAM132A
Uniprot No
Q5T7M4
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 85-89 | |
| 1:2 | Average % | 99 | |
| Range % | 91-102 | |
| 1:4 | Average % | 100 | |
| Range % | 93-108 | |
| 1:8 | Average % | 94 | |
| Range % | 90-99 | |
回收率
| The recovery of human FAM132A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-96 | |
| EDTA plasma (n=4) | 94 | 90-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.380 | 2.356 | 2.368 | 2.237 | | | 1000 | 1.815 | 1.776 | 1.796 | 1.665 | | | 500 | 1.220 | 1.198 | 1.209 | 1.078 | | | 250 | 0.669 | 0.666 | 0.668 | 0.537 | | | 125 | 0.438 | 0.424 | 0.431 | 0.300 | | | 62.5 | 0.226 | 0.222 | 0.224 | 0.093 | | | 31.25 | 0.181 | 0.178 | 0.180 | 0.049 | | | 0 | 0.134 | 0.128 | 0.131 | | |
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货期
3-5 working days