产品描述
This Human DHEA-S ELISA Kit was designed for the quantitative measurement of Human DHEA-S protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 20 ng/mL-4000 ng/mL and the sensitivity is 10 ng/mL.
缩写
DHEA-S
灵敏度
10 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 90 | | |
| Range % | 85-103 | | |
| 1:2 | Average % | 96 | | |
| Range % | 89-105 | | |
| 1:4 | Average % | 94 | | |
| Range % | 90-100 | | |
| 1:8 | Average % | 90 | | |
| Range % | 83-110 | | |
回收率
| The recovery of human DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 86-103 | | |
| EDTA plasma (n=4) | 89 | 84-93 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | | | | | 0 | 1.953 | 1.917 | 1.935 | | | | | 20 | 1.321 | 1.339 | 1.330 | | | | | 80 | 0.843 | 0.855 | 0.849 | | | | | 320 | 0.511 | 0.530 | 0.521 | | | | | 1000 | 0.329 | 0.324 | 0.327 | | | | | 4000 | 0.189 | 0.184 | 0.187 | | | |
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货期
3-5 working days