产品描述
This Human GSTO1 ELISA Kit was designed for the quantitative measurement of Human GSTO1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.2 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
AA407097 ELISA Kit; AI194287 ELISA Kit; AU018802 ELISA Kit; DKFZp686H13163 ELISA Kit; EC 2.5.1.18 ELISA Kit; Glutathione S transferase omega 1 ELISA Kit; Glutathione S-transferase omega 1-1 ELISA Kit; Glutathione S-transferase omega ELISA Kit; Glutathione S-transferase omega-1 ELISA Kit; Glutathione transferase omega 1 ELISA Kit; Glutathione-dependent dehydroascorbate reductase ELISA Kit; Glutathione-S-transferase like ELISA Kit; Glutathione-S-transferase omega 1 ELISA Kit; GSTO 1-1 ELISA Kit; GSTO-1 ELISA Kit; GSTO1 1 ELISA Kit; Gsto1 ELISA Kit; GSTO1_HUMAN ELISA Kit; GSTTLp28 ELISA Kit; GSTX ELISA Kit; Gtsttl ELISA Kit; MGC94845 ELISA Kit; MMA(V) reductase ELISA Kit; Monomethylarsonic acid reductase ELISA Kit; OTTHUMP00000020429 ELISA Kit; p28 ELISA Kit; S-(Phenacyl)glutathione reductase ELISA Kit; SPG-R ELISA Kit
缩写
GSTO1
Uniprot No
P78417
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Tags & Cell Markers
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GSTO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
83-94 |
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| 1:2 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:4 |
Average % |
93 |
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| Range % |
86-97 |
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| 1:8 |
Average % |
104 |
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| Range % |
97-108 |
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回收率
| The recovery of human GSTO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
87-96 |
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| EDTA plasma (n=4) |
89 |
85-94 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.304 |
2.281 |
2.293 |
2.117 |
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| 1000 |
1.453 |
1.373 |
1.413 |
1.237 |
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| 500 |
0.888 |
0.862 |
0.875 |
0.699 |
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| 250 |
0.534 |
0.520 |
0.527 |
0.351 |
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| 125 |
0.395 |
0.383 |
0.389 |
0.213 |
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| 62.5 |
0.292 |
0.285 |
0.289 |
0.113 |
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| 31.2 |
0.246 |
0.238 |
0.242 |
0.066 |
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| 0 |
0.178 |
0.173 |
0.176 |
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货期
3-5 working days