产品描述
This Guinea pig IL8 ELISA Kit was designed for the quantitative measurement of Guinea pig IL8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant protein 1 ELISA Kit; NAP-1 ELISA Kit
缩写
CXCL8
Uniprot No
P49113
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 90-102 | |
| 1:2 | Average % | 95 | |
| Range % | 85-100 | |
| 1:4 | Average % | 95 | |
| Range % | 88-102 | |
| 1:8 | Average % | 97 | |
| Range % | 90-104 | |
回收率
| The recovery of guinea pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 95-102 | |
| EDTA plasma (n=4) | 94 | 90-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.253 | 2.245 | 2.249 | 2.134 | | | 1000 | 1.631 | 1.696 | 1.664 | 1.549 | | | 500 | 1.111 | 1.135 | 1.123 | 1.008 | | | 250 | 0.717 | 0.752 | 0.735 | 0.620 | | | 125 | 0.434 | 0.423 | 0.429 | 0.314 | | | 62.5 | 0.343 | 0.354 | 0.349 | 0.234 | | | 31.25 | 0.228 | 0.241 | 0.235 | 0.120 | | | 0 | 0.116 | 0.113 | 0.115 | | |
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货期
3-5 working days