产品描述

This Human IGF2BP2 ELISA Kit was designed for the quantitative measurement of Human IGF2BP2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

别名

Hepatocellular carcinoma autoantigen p62 ELISA Kit; IF2B2_HUMAN ELISA Kit; IGF 2 mRNA binding protein 2 ELISA Kit; IGF II mRNA binding protein 2 ELISA Kit; IGF-II mRNA-binding protein 2 ELISA Kit; IGF2 mRNA-binding protein 2 ELISA Kit; Igf2bp2 ELISA Kit; IMP 2 ELISA Kit; IMP-2 ELISA Kit; IMP2 ELISA Kit; Insulin like growth factor 2 mRNA binding protein 2 ELISA Kit; Insulin-like growth factor 2 mRNA-binding protein 2 ELISA Kit; OTTHUMP00000082606 ELISA Kit; OTTHUMP00000082607 ELISA Kit; OTTHUMP00000082608 ELISA Kit; p62 ELISA Kit; VICKZ 2 ELISA Kit; VICKZ family member 2 ELISA Kit; VICKZ2 ELISA Kit

缩写

IGF2BP2

Uniprot No

Q9Y6M1

灵敏度

31.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IGF2BP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-90
1:2		Average %		96
		Range %		92-100
1:4		Average %		90
		Range %		85-94
1:8		Average %		104
		Range %		100-108

回收率

The recovery of human IGF2BP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		84-93
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 8000 2.167 2.249 2.208 2.051 4000 1.649 1.703 1.676 1.519 2000 1.174 1.114 1.144 0.987 1000 0.791 0.758 0.775 0.618 500 0.458 0.472 0.465 0.308 250 0.335 0.347 0.341 0.184 125 0.226 0.236 0.231 0.074 0 0.153 0.160 0.157

货期

3-5 working days