产品描述
This Human HNP1-3 ELISA Kit was designed for the quantitative measurement of Human HNP1-3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.
缩写
HNP1-3
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HNP1-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 92-102 | |
| 1:2 | Average % | 85 | |
| Range % | 81-90 | |
| 1:4 | Average % | 95 | |
| Range % | 87-99 | |
| 1:8 | Average % | 100 | |
| Range % | 85-106 | |
回收率
| The recovery of human HNP1-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 85-93 | |
| EDTA plasma (n=4) | 95 | 87-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.800 | 2.788 | 2.794 | 2.692 | | | 50 | 1.765 | 1.743 | 1.754 | 1.652 | | | 25 | 1.215 | 1.203 | 1.209 | 1.107 | | | 12.5 | 0.698 | 0.678 | 0.688 | 0.586 | | | 6.25 | 0.362 | 0.355 | 0.359 | 0.257 | | | 3.12 | 0.262 | 0.246 | 0.254 | 0.152 | | | 1.56 | 0.169 | 0.156 | 0.163 | 0.061 | | | 0 | 0.104 | 0.100 | 0.102 | | |
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货期
3-5 working days