产品描述
生长分化因子15(Growth differentiation factor 15,GDF15)是一种细胞因子,属于TGF-β超家族。GDF15广泛存在于多种组织和细胞中,包括神经元、心肌细胞、肝细胞、脂肪细胞等。GDF15参与多种生物学过程,如细胞增殖、凋亡、炎症反应和代谢调节等。在许多疾病中,如心血管疾病、糖尿病、肥胖症等,GDF15的含量显著升高,因此GDF15被认为是一种潜在的生物标志物,可用于这些疾病的早期诊断和治疗监测。
华美生物所提供的Human growth differentiation factor 15(GDF15)ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆、尿液样本中的GDF15,其灵敏度为1.95 pg/mL,检测范围为7.8 pg/mL-500 pg/mL。
别名
GDF 15 ELISA Kit; GDF-15 ELISA Kit; Gdf15 ELISA Kit; GDF15_HUMAN ELISA Kit; Growth differentiation factor 15 ELISA Kit; Growth/differentiation factor 15 ELISA Kit; Macrophage inhibitory cytokine 1 ELISA Kit; MIC 1 ELISA Kit; Mic-1 ELISA Kit; MIC1 ELISA Kit; NAG 1 ELISA Kit; NAG-1 ELISA Kit; NAG1 ELISA Kit; NRG 1 ELISA Kit; NRG-1 ELISA Kit; NRG1 ELISA Kit; NSAID (nonsteroidal anti inflammatory drug) activated protein 1 ELISA Kit; NSAID ELISA Kit; NSAID regulated protein 1 ELISA Kit; NSAID-activated gene 1 protein ELISA Kit; NSAID-regulated gene 1 protein ELISA Kit; PDF ELISA Kit; PLAB ELISA Kit; Placental bone morphogenetic protein ELISA Kit; Placental bone morphogenic protein ELISA Kit; Placental TGF beta ELISA Kit; Placental TGF-beta ELISA Kit; Prostate differentiation factor ELISA Kit; PTGF beta ELISA Kit; PTGFB ELISA Kit
缩写
GDF15
Uniprot No
Q99988
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GDF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
80-91 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-104 |
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| 1:4 |
Average % |
97 |
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| Range % |
90-105 |
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| 1:8 |
Average % |
95 |
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| Range % |
91-100 |
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回收率
| The recovery of human GDF15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
86-99 |
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| EDTA plasma (n=4) |
97 |
91-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
1.756 |
1.737 |
1.747 |
1.601 |
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| 250 |
1.134 |
1.142 |
1.138 |
0.992 |
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| 125 |
0.702 |
0.719 |
0.711 |
0.565 |
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| 62.5 |
0.465 |
0.450 |
0.458 |
0.312 |
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| 31.2 |
0.337 |
0.349 |
0.343 |
0.197 |
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| 15.6 |
0.266 |
0.272 |
0.269 |
0.123 |
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| 7.8 |
0.192 |
0.203 |
0.198 |
0.052 |
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| 0 |
0.145 |
0.147 |
0.146 |
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货期
3-5 working days