产品描述
The mouse IFNB (IFNB) ELISA Kit quantitates mouse IFNB levels in multiple samples, including serum, plasma, cell culture supernates, tissue homogenates, and cell lysates. IFNB is an essential cytokine in promoting and regulating innate and adaptive immune responses. It has both anti-inflammatory and pro-inflammatory properties. IFNB binds to IFNAR1 and IFNAR2 and then activates JAK/STAT pathway, leading to the phosphorylation of STAT1 and STAT2 and the formation of the STAT1/STAT2/IRF3 complex, finally stimulating the expression of genes that encodes proteins exerting antiviral, antiproliferative, and antitumor effects. IFNB has been applied to multiple sclerosis treatment via the downregulation of the MHC class II expression in antigen-presenting cells, the induction of IL-10 synthesis, and the suppression of T cell migration. The antiviral and immunoregulatory effects of IFNB could be most effective if used in the early stages of COVID-19.
This kit employs the sandwich-ELISA mechanism in conjugation with IFNB antibody-IFNB antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of IFNB in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%.
别名
Ifnb1 ELISA Kit; Ifb ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
缩写
IFNB1
Uniprot No
P01575
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-β/IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 90-103 | |
| 1:2 | Average % | 89 | |
| Range % | 84-95 | |
| 1:4 | Average % | 100 | |
| Range % | 95-106 | |
| 1:8 | Average % | 97 | |
| Range % | 92-104 | |
回收率
| The recovery of mouse IFN-β/IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 93-96 | |
| EDTA plasma (n=4) | 100 | 95-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.516 | 2.568 | 2.542 | 2.437 | | | 500 | 2.115 | 2.135 | 2.125 | 2.020 | | | 250 | 1.401 | 1.435 | 1.418 | 1.313 | | | 125 | 0.761 | 0.730 | 0.746 | 0.641 | | | 62.5 | 0.343 | 0.369 | 0.356 | 0.251 | | | 31.2 | 0.242 | 0.253 | 0.248 | 0.143 | | | 15.6 | 0.179 | 0.172 | 0.176 | 0.071 | | | 0 | 0.104 | 0.106 | 0.105 | | |
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货期
3-5 working days
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