产品描述
This Pig Haptoglobin (HP) ELISA Kit was designed for the quantitative measurement of Pig Haptoglobin (HP) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 μg/mL-100 μg/mL and the sensitivity is 0.39 μg/mL.
别名
HP ELISA Kit; Haptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
缩写
HP
Uniprot No
Q8SPS7
灵敏度
0.39 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Hpt/HP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
93 |
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| Range % |
89-98 |
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| 1:40 |
Average % |
98 |
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| Range % |
93-103 |
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| 1:80 |
Average % |
95 |
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| Range % |
89-98 |
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| 1:160 |
Average % |
94 |
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| Range % |
87-98 |
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回收率
| The recovery of pig Hpt/HP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
94-102 |
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| EDTA plasma (n=4) |
87 |
82-94 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ug/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
1.812 |
1.724 |
1.768 |
1.625 |
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| 50 |
1.145 |
1.212 |
1.179 |
1.036 |
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| 25 |
0.715 |
0.724 |
0.720 |
0.577 |
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| 12.5 |
0.451 |
0.438 |
0.445 |
0.302 |
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| 6.25 |
0.312 |
0.322 |
0.317 |
0.174 |
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| 3.12 |
0.255 |
0.242 |
0.249 |
0.106 |
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| 1.56 |
0.196 |
0.190 |
0.193 |
0.050 |
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| 0 |
0.142 |
0.144 |
0.143 |
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货期
3-5 working days