产品描述
This Mouse Eotaxin 3 ELISA Kit was designed for the quantitative measurement of Mouse Eotaxin 3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
缩写
Eotaxin 3
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Eotaxin 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 90-93 | |
| 1:2 | Average % | 96 | |
| Range % | 92-100 | |
| 1:4 | Average % | 103 | |
| Range % | 101-105 | |
| 1:8 | Average % | 89 | |
| Range % | 84-94 | |
回收率
| The recovery of mouse Eotaxin 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 90-94 | |
| EDTA plasma (n=4) | 89 | 85-94 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.927 | 2.874 | 2.901 | 2.745 | | | 200 | 2.015 | 2.135 | 2.075 | 1.919 | | | 100 | 1.303 | 1.403 | 1.353 | 1.197 | | | 50 | 0.801 | 0.822 | 0.812 | 0.656 | | | 25 | 0.532 | 0.524 | 0.528 | 0.372 | | | 12.5 | 0.319 | 0.332 | 0.326 | 0.170 | | | 6.25 | 0.244 | 0.254 | 0.249 | 0.093 | | | 0 | 0.151 | 0.160 | 0.156 | | |
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货期
3-5 working days