产品描述
This Rat Glp1 ELISA Kit was designed for the quantitative measurement of Rat Glp1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL .
别名
N/A
缩写
Glp1
灵敏度
0.156 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 86 | |
| Range % | 84-95 | |
| 1:200 | Average % | 95 | |
| Range % | 91-100 | |
| 1:400 | Average % | 84 | |
| Range % | 81-89 | |
| 1:800 | Average % | 92 | |
| Range % | 86-98 | |
回收率
| The recovery of rat GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 87-96 | |
| EDTA plasma (n=4) | 103 | 95-108 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.607 | 2.744 | 2.676 | 2.531 | | | 20 | 2.303 | 2.451 | 2.377 | 2.232 | | | 10 | 1.817 | 1.922 | 1.870 | 1.725 | | | 5 | 1.133 | 1.191 | 1.162 | 1.017 | | | 2.5 | 0.621 | 0.632 | 0.627 | 0.482 | | | 1.25 | 0.344 | 0.355 | 0.350 | 0.205 | | | 0.625 | 0.234 | 0.248 | 0.241 | 0.096 | | | 0 | 0.140 | 0.150 | 0.145 | | |
|
货期
3-5 working days