产品描述

This Human GPI ELISA Kit was designed for the quantitative measurement of Human GPI protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

别名

AMF ELISA Kit; Aurocrine motility factor ELISA Kit; Autocrine motility factor ELISA Kit; DKFZp686C13233 ELISA Kit; EC 5.3.1.9 ELISA Kit; G6PI_HUMAN ELISA Kit; Glucose phosphate isomerase ELISA Kit; Glucose-6-phosphate isomerase ELISA Kit; GNPI ELISA Kit; GPI ELISA Kit; Gpi1 ELISA Kit; Hexose monophosphate isomerase ELISA Kit; Hexosephosphate isomerase ELISA Kit; Neuroleukin ELISA Kit; NLK ELISA Kit; Oxoisomerase ELISA Kit; PGI ELISA Kit; PHI ELISA Kit; Phosphoglucose isomerase ELISA Kit; Phosphohexomutase ELISA Kit; Phosphohexose isomerase ELISA Kit; Phosphosaccharomutase ELISA Kit; SA 36 ELISA Kit; SA-36 ELISA Kit; SA36 ELISA Kit; Sperm antigen 36 ELISA Kit

缩写

GPI

Uniprot No

P06744

灵敏度

3.12 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human GPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-95
1:2		Average %		102
		Range %		97-107
1:4		Average %		91
		Range %		85-97
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human GPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		84-95
EDTA plasma (n=4)		97		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 800 2.065 2.076 2.071 1.968 400 1.580 1.557 1.569 1.466 200 0.835 0.824 0.830 0.727 100 0.432 0.445 0.439 0.336 50 0.285 0.278 0.282 0.179 25 0.190 0.198 0.194 0.091 12.5 0.139 0.133 0.136 0.033 0 0.102 0.104 0.103

货期

3-5 working days