产品描述
This Human LAMP2 ELISA Kit was designed for the quantitative measurement of Human LAMP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
LAMP2; Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; LGP-96; CD antigen CD107b
缩写
HLAMP-2
Uniprot No
P13473
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays):CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HLAMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 90 | | |
| Range % | 85-97 | | |
| 1:2 | Average % | 104 | | |
| Range % | 100-110 | | |
| 1:4 | Average % | 89 | | |
| Range % | 84-93 | | |
| 1:8 | Average % | 96 | | |
| Range % | 91-100 | | |
回收率
| The recovery of human HLAMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 87 | 82-91 | | |
| EDTA plasma (n=4) | 93 | 89-97 | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.482 | 2.526 | 2.504 | 2.320 | | | | 10 | 1.870 | 1.912 | 1.891 | 1.707 | | | | 5 | 1.145 | 1.169 | 1.157 | 0.973 | | | | 2.5 | 0.715 | 0.693 | 0.704 | 0.520 | | | | 1.25 | 0.410 | 0.440 | 0.425 | 0.241 | | | | 0.625 | 0.290 | 0.271 | 0.281 | 0.097 | | | | 0.312 | 0.224 | 0.237 | 0.231 | 0.047 | | | | 0 | 0.180 | 0.188 | 0.184 | | | |
|
货期
3-5 working days