产品描述

This Human FCGR3B ELISA Kit was designed for the quantitative measurement of Human FCGR3B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

别名

CD16 ELISA Kit; CD16b ELISA Kit; CD16b antigen ELISA Kit; Fc fragment of IgG low affinity IIIb receptor ELISA Kit; Fc gamma receptor IIIb ELISA Kit; Fc gamma RIII ELISA Kit; Fc gamma RIII beta ELISA Kit; Fc gamma RIIIb ELISA Kit; Fc-gamma RIII ELISA Kit; Fc-gamma RIII-beta ELISA Kit; Fc-gamma RIIIb ELISA Kit; FCG3 ELISA Kit; FCG3B_HUMAN ELISA Kit; FCGR3 ELISA Kit; FCGR3B ELISA Kit; FcR 10 ELISA Kit; FcR-10 ELISA Kit; FcR10 ELISA Kit; FcRIII ELISA Kit; FcRIIIb ELISA Kit; IGFR3 ELISA Kit; IgG Fc receptor III 1 ELISA Kit; IgG Fc receptor III-1 ELISA Kit; Low affinity immunoglobulin gamma Fc region receptor III B ELISA Kit; Low affinity immunoglobulin gamma Fc region receptor III-B ELISA Kit

缩写

FCGR3B

Uniprot No

O75015

灵敏度

0.39 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human FCGR3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:20		Average %		92
		Range %		86-100
1:40		Average %		97
		Range %		90-104
1:80		Average %		95
		Range %		87-99
1:160		Average %		93
		Range %		90-99

回收率

The recovery of human FCGR3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		87-98
EDTA plasma (n=4)		93		90-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 100 2.988 2.875 2.932 2.714 50 2.448 2.396 2.422 2.204 25 1.678 1.716 1.697 1.479 12.5 1.025 1.043 1.034 0.816 6.25 0.670 0.651 0.661 0.443 3.12 0.473 0.462 0.468 0.250 1.56 0.321 0.318 0.320 0.102 0 0.219 0.216 0.218

货期

3-5 working days