产品描述
This Pig HSP70 ELISA Kit was designed for the quantitative measurement of Pig HSP70 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
别名
HSPA1A ELISA Kit; HSPA1Heat shock 70 kDa protein 1A ELISA Kit; Heat shock 70 kDa protein 1 ELISA Kit; HSP70.1 ELISA Kit
缩写
HSP-70
Uniprot No
P34930
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
92 |
|
| Range % |
89-95 |
|
| 1:2 |
Average % |
89 |
|
| Range % |
83-94 |
|
| 1:4 |
Average % |
92 |
|
| Range % |
87-100 |
|
| 1:8 |
Average % |
94 |
|
| Range % |
89-107 |
|
回收率
| The recovery of pig HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
87-99 |
|
| EDTA plasma (n=4) |
90 |
85-96 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 40 |
1.952 |
1.931 |
1.942 |
1.807 |
|
| 20 |
1.570 |
1.613 |
1.592 |
1.457 |
|
| 10 |
1.235 |
1.268 |
1.252 |
1.117 |
|
| 5 |
0.911 |
0.897 |
0.904 |
0.769 |
|
| 2.5 |
0.672 |
0.656 |
0.664 |
0.529 |
|
| 1.25 |
0.409 |
0.395 |
0.402 |
0.267 |
|
| 0.625 |
0.265 |
0.251 |
0.258 |
0.123 |
|
| 0 |
0.138 |
0.131 |
0.135 |
|
|
|
货期
3-5 working days