产品描述

This Human GPC3 ELISA Kit was designed for the quantitative measurement of Human GPC3 protein in serum, urine, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

DGSX ELISA Kit; Glypican proteoglycan 3 ELISA Kit; Glypican-3 [Precursor] ELISA Kit; Gpc3 ELISA Kit; GPC3_HUMAN ELISA Kit; GTR2 2 ELISA Kit; GTR2-2 ELISA Kit; Heparan sulphate proteoglycan ELISA Kit; Intestinal protein OCI 5 ELISA Kit; Intestinal protein OCI-5 ELISA Kit; MXR7 ELISA Kit; OCI 5 ELISA Kit; OCI-5 ELISA Kit; OCI5 ELISA Kit; SDYS ELISA Kit; Secreted glypican-3 ELISA Kit; SGB ELISA Kit; SGBS ELISA Kit; SGBS1 ELISA Kit

缩写

GPC3

Uniprot No

P51654

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human GPC-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		80-100
1:2		Average %		98
		Range %		91-105
1:4		Average %		100
		Range %		92-110
1:8		Average %		93
		Range %		86-98

回收率

The recovery of human GPC-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.621 2.641 2.631 2.437 20 2.356 2.194 2.275 2.081 10 1.626 1.674 1.650 1.456 5 1.021 1.101 1.061 0.867 2.5 0.596 0.600 0.598 0.404 1.25 0.412 0.422 0.417 0.223 0.625 0.302 0.315 0.309 0.115 0 0.187 0.201 0.194

货期

3-5 working days