产品描述
This Mouse HMOX1 ELISA Kit was designed for the quantitative measurement of Mouse HMOX1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
Hmox1 ELISA Kit; Heme oxygenase 1 ELISA Kit; HO-1 ELISA Kit; EC 1.14.14.18 ELISA Kit; P32 protein ELISA Kit
缩写
HMOX1
Uniprot No
P14901
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 92 | |
| Range % | 89-96 | |
| 1:200 | Average % | 96 | |
| Range % | 91-101 | |
| 1:400 | Average % | 97 | |
| Range % | 93-102 | |
| 1:800 | Average % | 93 | |
| Range % | 87-99 | |
回收率
| The recovery of mouse HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 93-101 | |
| EDTA plasma (n=4) | 91 | 85-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.416 | 2.312 | 2.364 | 2.173 | | | 1000 | 1.827 | 1.906 | 1.867 | 1.676 | | | 500 | 1.247 | 1.265 | 1.256 | 1.065 | | | 250 | 0.799 | 0.829 | 0.814 | 0.623 | | | 125 | 0.477 | 0.486 | 0.482 | 0.291 | | | 62.5 | 0.334 | 0.362 | 0.348 | 0.157 | | | 31.25 | 0.257 | 0.269 | 0.263 | 0.072 | | | 0 | 0.194 | 0.188 | 0.191 | | |
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货期
3-5 working days