产品描述
This Rat GPⅡbⅢa/CD41+CD61 ELISA Kit was designed for the quantitative measurement of Rat GPⅡbⅢa/CD41+CD61 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.
别名
platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa |platelet membrane glycoprotein 2b3a,GP-2b3a
缩写
GPⅡbⅢa/CD41+CD61
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GP-ⅡbⅢa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 82-92 | |
| 1:2 | Average % | 89 | |
| Range % | 82-95 | |
| 1:4 | Average % | 95 | |
| Range % | 90-100 | |
| 1:8 | Average % | 90 | |
| Range % | 85-98 | |
回收率
| The recovery of rat GP-ⅡbⅢa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-97 | |
| EDTA plasma (n=4) | 98 | 92-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.176 | 2.053 | 2.115 | 1.921 | | | 200 | 1.855 | 1.764 | 1.810 | 1.616 | | | 100 | 1.542 | 1.469 | 1.506 | 1.312 | | | 50 | 1.236 | 1.178 | 1.207 | 1.013 | | | 25 | 0.837 | 0.786 | 0.812 | 0.618 | | | 12.5 | 0.421 | 0.393 | 0.407 | 0.213 | | | 6.25 | 0.213 | 0.196 | 0.205 | 0.011 | | | 0 | 0.198 | 0.190 | 0.194 | | |
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货期
3-5 working days