产品描述
This Human FRA ELISA Kit was designed for the quantitative measurement of Human FRA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 μmol/mL-12 μmol/mL and the sensitivity is 0.04 μmol/mL .
缩写
FRA
灵敏度
0.04 μmol/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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回收率
| The recovery of human FRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 88-96 | | |
| EDTA plasma (n=4) | 98 | 95-102 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| μmol/ml | OD1 | OD2 | Average | Corrected | | | | 12 | 2.452 | 2.522 | 2.487 | 2.313 | | | | 6 | 2.063 | 2.031 | 2.047 | 1.873 | | | | 3 | 1.444 | 1.512 | 1.478 | 1.304 | | | | 1.5 | 0.874 | 0.899 | 0.887 | 0.713 | | | | 0.75 | 0.512 | 0.545 | 0.529 | 0.355 | | | | 0.375 | 0.351 | 0.365 | 0.358 | 0.184 | | | | 0.18 | 0.275 | 0.255 | 0.265 | 0.091 | | | | 0 | 0.167 | 0.180 | 0.174 | | | |
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货期
3-5 working days