产品描述

This Human GPT ELISA Kit was designed for the quantitative measurement of Human GPT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 uIU/mL-1000 uIU/mL and the sensitivity is 3.9 uIU/mL.

别名

AAT1 ELISA Kit; Alanine aminotransferase 1 ELISA Kit; Alanine aminotransferase ELISA Kit; ALAT1_HUMAN ELISA Kit; ALT1 ELISA Kit; Glutamate pyruvate transaminase 1 ELISA Kit; Glutamic alanine transaminase 1 ELISA Kit; Glutamic pyruvate transaminase (alanine aminotransferase) ELISA Kit; Glutamic pyruvic transaminase 1 ELISA Kit; Glutamic--alanine transaminase 1 ELISA Kit; Glutamic--pyruvic transaminase 1 ELISA Kit; GPT 1 ELISA Kit; gpt ELISA Kit; GPT1 ELISA Kit

缩写

GPT

Uniprot No

P24298

灵敏度

3.9 uIU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ALT/GPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		98
		Range %		92-104
1:2		Average %		84
		Range %		80-88
1:4		Average %		94
		Range %		90-98
1:8		Average %		92
		Range %		87-96

回收率

The recovery of human ALT/GPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-94
EDTA plasma (n=4)		96		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

uIU/ml OD1 OD2 Average Corrected   1000 2.542 2.687 2.615 2.516   500 1.706 1.717 1.712 1.613   250 1.003 1.089 1.046 0.947   125 0.626 0.597 0.612 0.513   62.5 0.317 0.304 0.311 0.212   31.2 0.255 0.239 0.247 0.148   15.6 0.185 0.171 0.178 0.079   0 0.103 0.095 0.099

货期

3-5 working days