产品描述
The product CSB-EL006252MO is a sandwich ELISA kit developed to measure concentrations of mouse CXCL9 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CXCL9 in samples. The color intensity is measured at 450 nm via a microplate reader.
CXCL9, also known as MIG, can be produced during inflammatory conditions by myeloid cells within the tumor microenvironment. It attracts CXCR3-expressing cells including activated T and NK cells to the inflammatory environment and plays a role in responses to immune checkpoint therapy. Overexpression of CXCL9 has also been shown to reduce tumor progression and metastasis via the inhibition of angiogenesis. However, CXCL9 can act directly on CXCR3-expressing tumor cells to promote cell migration and epithelial-mesenchymal transition.
别名
Cxcl9 ELISA Kit; Mig ELISA Kit; Scyb9C-X-C motif chemokine 9 ELISA Kit; Gamma-interferon-induced monokine ELISA Kit; Monokine induced by interferon-gamma ELISA Kit; MIG ELISA Kit; MuMIG ELISA Kit; Protein m119 ELISA Kit; Small-inducible cytokine B9 ELISA Kit
缩写
CXCL9
Uniprot No
P18340
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 85 | | |
| Range % | 82-89 | | |
| 1:2 | Average % | 106 | | |
| Range % | 103-109 | | |
| 1:4 | Average % | 89 | | |
| Range % | 85-93 | | |
| 1:8 | Average % | 107 | | |
| Range % | 103-110 | | |
回收率
| The recovery of mouse CXCL9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 96 | 93-99 | | |
| EDTA plasma (n=4) | 102 | 98-106 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.445 | 2.401 | 2.423 | 2.287 | | | | 500 | 1.938 | 1.905 | 1.922 | 1.786 | | | | 250 | 1.206 | 1.301 | 1.254 | 1.118 | | | | 125 | 0.667 | 0.609 | 0.638 | 0.502 | | | | 62.5 | 0.405 | 0.394 | 0.400 | 0.264 | | | | 31.2 | 0.256 | 0.258 | 0.257 | 0.121 | | | | 15.6 | 0.209 | 0.201 | 0.205 | 0.069 | | | | 0 | 0.137 | 0.134 | 0.136 | | | |
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货期
3-5 working days