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CXCL9小鼠C-X-C趋化因子9(CXCL9)酶联免疫试剂盒Mouse C-X-C motif chemokine 9(CXCL9) ELISA kit
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数万篇文献的选择,荣登各大SCI高分期刊
原料试剂 研发实验室
价格
¥2500.00-3600.00
品牌 华美生物/CUSABIO
地区 中国,湖北省,武汉市
货号 CSB-EL006252MO
产地 国产
选择规格
96T
48T
易享客服
武汉华美生物工程有限公司
武汉华美生物工程有限公司
湖北省武汉东湖新技术开发区神墩五路269号生之源股份生物科创产业园B栋
营业执照已审核
武汉华美生物工程有限公司成立于2007年12月,是一家以科研试剂生产、诊断试剂原料供应为核心,集自主研发、生产、营销为一体的生物高新技术企业。华美生物拥有多项先进的核心技术,致力于为全球高等院校、政府研究机构、诊断试剂生产厂家以及生物制药公司提供高质量产品与高水平的技术服务。 华美生物子品牌CUSABIO主要经营科研试剂产品,现拥有各物种基因产品一万余种;现货重组蛋白16,000多种;数十个种属的多克隆抗体65,000多种和千余种高质量单克隆抗体。ELISA试剂盒现已涵盖20多个种属、3,000个靶标、4,000多种产品,涉及肿瘤、激素、自身免疫、心血管、代谢等众多研究领域。华美CUSABIO产品远销欧美日德等九十多个国家和地区,已被众多国际顶级学术期刊Nature, Cell, Immunity, Nature Medicine, Cell Metabolism, Gastroenterology等引用。 华美生物总部位于湖北武汉,在美国建立了子公司,并在全球近百个国家拥有授权经销商。华美生物始终秉承“诚信与品质”的核心理念,借助自身的创新实力,匠心打造精品科研试剂与诊断原料,无畏前行,尽情展现着开创者的梦想与未来,为人类健康事业创造价值!
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产品规格 图文详情 技术文档
产品规格
品牌名称
华美生物/CUSABIO
货号
CSB-EL006252MO
国产/进口
国产
规格
96T,48T
图文详情

产品描述

The product CSB-EL006252MO is a sandwich ELISA kit developed to measure concentrations of mouse CXCL9 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CXCL9 in samples. The color intensity is measured at 450 nm via a microplate reader.

CXCL9, also known as MIG, can be produced during inflammatory conditions by myeloid cells within the tumor microenvironment. It attracts CXCR3-expressing cells including activated T and NK cells to the inflammatory environment and plays a role in responses to immune checkpoint therapy. Overexpression of CXCL9 has also been shown to reduce tumor progression and metastasis via the inhibition of angiogenesis. However, CXCL9 can act directly on CXCR3-expressing tumor cells to promote cell migration and epithelial-mesenchymal transition.


别名

Cxcl9 ELISA Kit; Mig ELISA Kit; Scyb9C-X-C motif chemokine 9 ELISA Kit; Gamma-interferon-induced monokine ELISA Kit; Monokine induced by interferon-gamma ELISA Kit; MIG ELISA Kit; MuMIG ELISA Kit; Protein m119 ELISA Kit; Small-inducible cytokine B9 ELISA Kit

缩写

CXCL9

Uniprot No

P18340

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 82-89
1:2 Average % 106
Range % 103-109
1:4 Average % 89
Range % 85-93
1:8 Average % 107
Range % 103-110

回收率

The recovery of mouse CXCL9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 93-99
EDTA plasma (n=4) 102 98-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.445 2.401 2.423 2.287
500 1.938 1.905 1.922 1.786
250 1.206 1.301 1.254 1.118
125 0.667 0.609 0.638 0.502
62.5 0.405 0.394 0.400 0.264
31.2 0.256 0.258 0.257 0.121
15.6 0.209 0.201 0.205 0.069
0 0.137 0.134 0.136  

货期

3-5 working days
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