产品描述
This Rabbit IL8 ELISA Kit was designed for the quantitative measurement of Rabbit IL8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.
别名
CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant/activation protein 1 ELISA Kit; NAP-1 ELISA Kit; Permeability factor 1 ELISA Kit; PF1 ELISA Kit; rPF1 ELISA Kit
缩写
CXCL8
Uniprot No
P19874
灵敏度
5.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 101 | |
| Range % | 94-105 | |
| 1:2 | Average % | 97 | |
| Range % | 91-102 | |
| 1:4 | Average % | 89 | |
| Range % | 85-94 | |
| 1:8 | Average % | 90 | |
| Range % | 86-98 | |
回收率
| The recovery of rabbit IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 87-96 | |
| EDTA plasma (n=4) | 92 | 89-94 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 2.390 | 2.345 | 2.368 | 2.285 | | | 750 | 1.587 | 1.476 | 1.532 | 1.449 | | | 375 | 0.814 | 0.778 | 0.796 | 0.713 | | | 187.5 | 0.409 | 0.389 | 0.399 | 0.316 | | | 94 | 0.276 | 0.264 | 0.270 | 0.187 | | | 47 | 0.182 | 0.172 | 0.177 | 0.094 | | | 23.5 | 0.134 | 0.131 | 0.133 | 0.050 | | | 0 | 0.085 | 0.081 | 0.083 | | |
|
货期
3-5 working days