产品描述

This Human EBAG9 ELISA Kit was designed for the quantitative measurement of Human EBAG9 protein in serum, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 U/mL-50 U/mL and the sensitivity is 0.195 U/mL .

别名

BAG9 ELISA Kit; Cancer associated surface antigen ELISA Kit; Cancer associated surface antigen RCAS1 ELISA Kit; Cancer-associated surface antigen RCAS1 ELISA Kit; EB9 ELISA Kit; EBAG 9 ELISA Kit; EBAG9 ELISA Kit; Estrogen receptor binding fragment associated gene 9 protein ELISA Kit; Estrogen receptor binding site associated antigen 9 ELISA Kit; estrogen receptor binding site associated; antigen; 9 ELISA Kit; Estrogen receptor-binding fragment-associated gene 9 protein ELISA Kit; PDAF ELISA Kit; RCAS 1 ELISA Kit; RCAS1 ELISA Kit; RCAS1_HUMAN ELISA Kit; Receptor binding cancer antigen expressed on SiSo cells ELISA Kit; Receptor-binding cancer antigen expressed on SiSo cells ELISA Kit

缩写

EBAG9

Uniprot No

O00559

灵敏度

0.195 U/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human RCAS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		90-98
1:2		Average %		96
		Range %		91-101
1:4		Average %		100
		Range %		95-106
1:8		Average %		105
		Range %		99-111

回收率

The recovery of human RCAS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		92-102
EDTA plasma (n=4)		100		95-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml OD1 OD2 Average Corrected 50 2.702 2.758 2.730 2.577 25 2.211 2.219 2.215 2.062 12.5 1.566 1.596 1.581 1.428 6.25 0.984 0.997 0.991 0.838 3.12 0.562 0.581 0.572 0.419 1.56 0.439 0.407 0.423 0.270 0.78 0.291 0.281 0.286 0.133 0 0.151 0.154 0.153

货期

3-5 working days