产品描述
This Bovine HSPA2 ELISA Kit was designed for the quantitative measurement of Bovine HSPA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .
别名
HSPA2 ELISA Kit; HSP70-3 ELISA Kit; HSPA3 ELISA Kit; Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit
缩写
HSPA2
Uniprot No
P34933
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSP-72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 87-98 | |
| 1:2 | Average % | 97 | |
| Range % | 92-105 | |
| 1:4 | Average % | 98 | |
| Range % | 94-108 | |
| 1:8 | Average % | 86 | |
| Range % | 82-92 | |
回收率
| The recovery of bovine HSP-72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 88-98 | |
| EDTA plasma (n=4) | 92 | 87-96 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.532 | 2.432 | 2.482 | 2.349 | | | 10 | 1.974 | 1.874 | 1.924 | 1.791 | | | 5 | 1.271 | 1.171 | 1.221 | 1.088 | | | 2.5 | 0.805 | 0.785 | 0.795 | 0.662 | | | 1.25 | 0.460 | 0.450 | 0.455 | 0.322 | | | 0.625 | 0.338 | 0.328 | 0.333 | 0.200 | | | 0.312 | 0.228 | 0.218 | 0.223 | 0.090 | | | 0 | 0.134 | 0.132 | 0.133 | | |
|
货期
3-5 working days