产品描述
This Deer IGF-1 ELISA Kit was designed for the quantitative measurement of Deer IGF-1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1 ng/mL-200 ng/mL and the sensitivity is 0.5 ng/mL .
缩写
IGF-1
Uniprot No
H9BDM1
灵敏度
0.5 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 92-104 | |
| 1:2 | Average % | 95 | |
| Range % | 89-104 | |
| 1:4 | Average % | 99 | |
| Range % | 87-107 | |
| 1:8 | Average % | 96 | |
| Range % | 90-102 | |
回收率
| The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-98 | |
| EDTA plasma (n=4) | 96 | 90-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | | | | 200 | 0.175 | 0.174 | 0.175 | | | | 50 | 0.333 | 0.316 | 0.325 | | | | 16 | 0.622 | 0.640 | 0.631 | | | | 4 | 1.040 | 1.048 | 1.044 | | | | 1 | 1.416 | 1.389 | 1.403 | | | | 0 | 2.577 | 2.633 | 2.605 | | |
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货期
3-5 working days