产品描述

The mouse neutrophil elastase (NE) ELISA Kit is engineered for accurate measurement of mouse NE levels from samples including serum, plasma, cell culture supernates, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the NE content in the sample. The color intensity is positively correlated with NE content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

NE is a serine protease that plays a causal role in inflammation and is involved in various physiological processes such as the formation of neutrophil extracellular trap and degradation of extracellular matrix and proteins. It is mainly found in neutrophil azurophilic granules. NE can be rapidly released from neutrophils in response to inflammatory stimuli and is therefore regarded as a sign of inflammation and is closely associated with some inflammatory diseases such as bronchiectasis and chronic obstructive pulmonary disease. It also actively participates in cardiovascular disease. NE enhances myocardial damage by triggering excessive inflammation. And many studies have found that high expression of NE in atherosclerotic plaques.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.849 2.793 2.821 2.627 10 2.132 2.222 2.177 1.983 5 1.465 1.388 1.427 1.233 2.5 0.948 0.929 0.939 0.745 1.25 0.643 0.666 0.655 0.461 0.625 0.472 0.442 0.457 0.263 0.312 0.278 0.288 0.283 0.089 0 0.198 0.189 0.194

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