产品描述
抑制素相关蛋白1(Dickkopf-1,DKK1)是一种由人类基因DKK1编码的蛋白质,是细胞外基质中的一种抑制素。DKK1通过与细胞膜上的Wnt受体相互作用,抑制Wnt信号通路的激活,进而影响细胞的增殖、分化和凋亡等生物学过程。近年来的研究发现,DKK1与多种肿瘤的发生和进展密切相关,可能成为肿瘤诊断和治疗的潜在靶点。
华美生物所提供的Human Dickkopf 1,DKK1 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的DKK1,其灵敏度为15.6 pg/mL,检测范围为62.5 pg/mL-4000pg/mL。
别名
Dickkopf 1 ELISA Kit; Dickkopf 1 homolog ELISA Kit; Dickkopf 1 like ELISA Kit; Dickkopf homolog 1 ELISA Kit; Dickkopf like protein 1 ELISA Kit; Dickkopf related protein 1 ELISA Kit; Dickkopf WNT signaling pathway inhibitor 1 ELISA Kit; Dickkopf-1 ELISA Kit; Dickkopf-related protein 1 ELISA Kit; DKK 1 ELISA Kit; Dkk-1 ELISA Kit; Dkk1 ELISA Kit; DKK1_HUMAN ELISA Kit; hDkk 1 ELISA Kit; hDkk-1 ELISA Kit; SK ELISA Kit
缩写
DKK1
Uniprot No
O94907
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human DKK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:2 |
Average % |
99 |
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| Range % |
92-102 |
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| 1:4 |
Average % |
100 |
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| Range % |
96-103 |
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| 1:8 |
Average % |
95 |
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| Range % |
88-98 |
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回收率
| The recovery of human DKK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-97 |
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| EDTA plasma (n=4) |
92 |
92-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.601 |
2.587 |
2.594 |
2.468 |
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| 2000 |
1.827 |
1.776 |
1.802 |
1.676 |
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| 1000 |
1.249 |
1.228 |
1.239 |
1.113 |
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| 500 |
0.743 |
0.735 |
0.739 |
0.613 |
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| 250 |
0.447 |
0.424 |
0.436 |
0.310 |
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| 125 |
0.274 |
0.266 |
0.270 |
0.144 |
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| 62.5 |
0.193 |
0.188 |
0.191 |
0.065 |
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| 0 |
0.128 |
0.124 |
0.126 |
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货期
3-5 working days