产品描述
This Human HELIXⅡ ELISA Kit was designed for the quantitative measurement of Human HELIXⅡ protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.312 ng/mL .
别名
type 2 collagen helical peptide,HELIX-2,_x000D_
type II collagen helical peptide,HELIX-II
缩写
HELIXⅡ
灵敏度
0.312 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HELIX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-99 |
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| 1:2 |
Average % |
89 |
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| Range % |
83-94 |
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| 1:4 |
Average % |
99 |
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| Range % |
94-103 |
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| 1:8 |
Average % |
104 |
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| Range % |
100-112 |
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回收率
| The recovery of human HELIX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
84 |
80-88 |
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| EDTA plasma (n=4) |
91 |
86-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
2.178 |
2.114 |
2.146 |
2.043 |
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| 40 |
1.674 |
1.581 |
1.628 |
1.525 |
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| 20 |
1.116 |
1.061 |
1.089 |
0.986 |
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| 10 |
0.626 |
0.603 |
0.615 |
0.512 |
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| 5 |
0.423 |
0.407 |
0.415 |
0.312 |
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| 2.5 |
0.252 |
0.239 |
0.246 |
0.143 |
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| 1.25 |
0.193 |
0.184 |
0.189 |
0.086 |
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| 0 |
0.104 |
0.101 |
0.103 |
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货期
3-5 working days